Lihat sumber bagi 6-Fosfoglukonat dehidrogenase

{{Pfam box
| Symbol = 6PGD
| Name = 6PGD
| image = PDB 1pgq EBI.jpg
| width = 
| caption = Struktur hablur 6-fosfoglukonat dehidrogenase bebiri sebagai kompleks dengan adenosina 2'-monofosfat<ref name="pmid7922042">{{PDB|1PGQ}}; {{cite journal | vauthors = Adams MJ, Ellis GH, Gover S, Naylor CE, Phillips C | title = Crystallographic study of coenzyme, coenzyme analogue and substrate binding in 6-phosphogluconate dehydrogenase: implications for NADP specificity and the enzyme mechanism | journal = Structure | volume = 2 | issue = 7 | pages = 651–68 |date=July 1994 | pmid = 7922042 | doi = 10.1016/s0969-2126(00)00066-6| doi-access =  }}</ref>
| Pfam = PF00393
| Pfam_clan = CL0106 
| InterPro = IPR006114
| SMART = 
| PROSITE = PDOC00390
| MEROPS = 
| SCOP = 2pgd
| TCDB = 
| OPM family = 
| OPM protein = 
| CAZy = 
| CDD = 
}}
{{infobox enzyme
| Name       = Fosfoglukonat dehidrogenase
| EC_number  = 1.1.1.44
| CAS_number = 9001-82-5
| GO_code    = 0008114
| image      = 1pgo.jpg
| width      = 270
| caption    = Dimer 6-fosfoglukonat dehidrogenase, ''Ovis aries''
}}
{{protein
|Name=Fosfoglukonat dehidrogenase
|caption=
|image=
|width=
|HGNCid=8891
|Symbol=PGD
|AltSymbols=
|EntrezGene=5226
|OMIM=172200
|RefSeq=NM_002631
|UniProt=P52209
|PDB=
|ECnumber=1.1.1.44
|Chromosome=1
|Arm=p
|Band=36.3
|LocusSupplementaryData=-36.13
}}
'''6-Fosfoglukonat dehidrogenase''' ('''6PGD''') ialah sebuah [[enzim]] dalam [[laluan pentosa fosfat]]. Ia membentuk [[ribulosa 5-fosfat]] daripada [[6-fosfoglukonat]]:

:6-fosfo-D-glukonat + NAD(P)<sup>+</sup> <math>\rightleftharpoons</math> D-Ribulosa 5-fosfat + CO2 + NAD(P)H + H<sup>+</sup>

Ia merupakan satu karboksilase oksidatif yang [[Pemangkinan|memangkinkan]] penyahkarboksilan [[Redoks|penurunan]] [[6-fosfoglukonat]] kepada [[ribulosa 5-fosfat]] dengan kehadiran [[NADP]]. Tindak balas ini ialah komponen bagi saluran [[heksosa]] monofosfat dan [[laluan pentosa fosfat]] (PPP).<ref name="pmid2113917">{{cite journal | vauthors = Broedel SE, Wolf RE | title = Genetic tagging, cloning, and DNA sequence of the Synechococcus sp. strain PCC 7942 gene (gnd) encoding 6-phosphogluconate dehydrogenase | journal = J. Bacteriol. | volume = 172 | issue = 7 | pages = 4023–31 |date=July 1990 | pmid = 2113917 | pmc = 213388 | doi = 10.1128/jb.172.7.4023-4031.1990}}</ref><ref name="pmid6641716">{{cite journal | vauthors = Adams MJ, Archibald IG, Bugg CE, Carne A, Gover S, [[John R. Helliwell|Helliwell JR]], Pickersgill RW, White SW | title = The three dimensional structure of sheep liver 6-phosphogluconate dehydrogenase at 2.6 A resolution | journal = EMBO J. | volume = 2 | issue = 6 | pages = 1009–14 | year = 1983 | pmid = 6641716 | pmc = 555222 | doi = 10.1002/j.1460-2075.1983.tb01535.x}}</ref> 6PGD prokariot dan eukariot ialah protein dengan panjang kira-kira 470 asid amino dengan jujukan yang amat terpelihara.<ref name="pmid1659648">{{cite journal | vauthors = Reizer A, Deutscher J, Saier MH, Reizer J | title = Analysis of the gluconate (gnt) operon of Bacillus subtilis | journal = Mol. Microbiol. | volume = 5 | issue = 5 | pages = 1081–9 |date=May 1991 | pmid = 1659648 | doi = 10.1111/j.1365-2958.1991.tb01880.x| s2cid = 2006623 }}</ref> Protein ialah [[homodimer]], di mana [[monomer]] bertindak secara bebas:<ref name="pmid6641716" /> setiap satunya mengandungi domain [[heliks alfa|alfa-heliks]] yang besar, dan domain beta-alfa-beta yang lebih kecil, mengandungi [[helaian beta]] selari dan antiselari yang bercampur-campur.<ref name="pmid6641716" /> NADP terikat dalam celah dalam domain kecil, dan [[substrat enzim|substrat]] pula mengikat di poket bersebelahan.<ref name="pmid6641716" />

== Kepentingan bioteknologi ==
Kini, 6PGD diperhatikan dapat memangkinkan tindak balas terbalik (iaitu [[pengkarboksilan]] penurunan) secara ''in vivo''.<ref>{{cite journal | vauthors = Satanowski A, Dronsella B, Noor E, Vögeli B, He H, Wichmann P, Erb TJ, Lindner SN, Bar-Even A | display-authors = 6 | title = Awakening a latent carbon fixation cycle in Escherichia coli | journal = Nature Communications | volume = 11 | issue = 1 | pages = 5812 | date = November 2020 | pmid = 33199707 | pmc = 7669889 | doi = 10.1038/s41467-020-19564-5 | bibcode = 2020NatCo..11.5812S }}</ref> Uji kaji dengan strain terpilih ''Escherichia coli'' menunjukkan bahawa tindak balas ini adalah mencukupi untuk menyokong membentukan [[biojisim]] berdasarkan [[Karbon dioksida|CO<sub>2</sub>]] dan [[Pentosa|gula pentosa]] semata-mata. Ciri ini diharapkan dapat dimanfaatkan bagi laluan [[penetapan karbon]] sintetik.

== Kepentingan klinikal ==

Mutasi dalam gen yang mengekod enzim ini mengakibatkan [[kekurangan 6-fosfoglukonat dehidrogenase]], penyakit keturunan [[autosom]] yang menjejaskan sel darah merah.

6PGD terlibat dalam metabolisme sel kanser, membawa kepada usaha untuk menghasilkan perencat 6PGD.<ref>[http://www.nature.com/ncb/journal/v17/n11/full/ncb3255.html 6-Phosphogluconate dehydrogenase links oxidative PPP, lipogenesis and tumour growth by inhibiting LKB1–AMPK signalling. 2015]</ref>

== Rujukan ==
{{Reflist}}

== Bacaan lanjut ==
{{refbegin}}
* {{cite journal | vauthors = Frampton EW, Wood WA | title = Carbohydrate oxidation by Pseudomonas fluorescens VI. Conversion of 2-keto-6-phosphogluconate to pyruvate | journal = The Journal of Biological Chemistry | volume = 236 | pages = 2571–7 | date = October 1961 | doi = 10.1016/S0021-9258(19)61700-X | pmid = 13894458 | doi-access = free }}
{{refend}}

{{InterPro content|IPR006114}}

{{Laluan pentosa fosfat}}
{{Oksidoreduktase alkohol}}
{{Portal bar|Biologi|border=no}}

[[Category:Enzim bergantungan NADPH]]
[[Category:Enzim bergantungan NADH]]
[[Category:EC 1.1.1]]